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MECHANISM OF ACTION
Optune Pax® delivers TTFields, which disrupt processes critical for cancer cell division and survival1,2
What are Tumor Treating Fields (TTFields)?
TTFields are alternating electric fields that target the electrical properties of cancer cells to disrupt cell viability without significantly affecting healthy cells.3-5
Healthy cell
T cell
Microtubule
Tumor cell
TTFields
Tubulin dimers
Disrupt mitosis4,6-8
As cancer cells divide rapidly, they are vulnerable to the antimitotic effects of TTFields. Preclinical evidence has shown that by exerting physical forces on electrically charged components of cancer cells, TTFields can disrupt mitotic processes, such as assembly of the mitotic spindle, leading to impaired cell division and downstream immunogenic cell death.
Downstream immune response4,9,10
Preclinical data showed that immunogenic cell death leads to expansion of T cells that target cancer-specific antigens, increased T-cell infiltration at the tumor site, and increased cancer cell surveillance throughout the body.
Why are healthy cells not significantly affected by Optune Pax?
Healthy cells have different properties (including division rate, morphology, and electrical properties) from cancer cells and, therefore, are not significantly affected by Optune Pax.3-5
Is Optune Pax right for your patients with locally advanced pancreatic cancer?
AE=adverse event.
References: 1. Optune Pax for Locally Advanced Pancreatic Cancer (LAPC). Physician Instructions for Use. Novocure; 2026. 2. Novocure Data on File 2025. [EF-27 (PANOVA-3) Study protocol.] 3. Vergote I, Macarulla T, Hirsch FR, Hagemann C, Miller DS. Tumor treating fields (TTFields) therapy concomitant with taxanes for cancer treatment. Cancers (Basel). 2023;15(3):636. doi:10.3390/cancers15030636 4. Karanam NK, Story MD. An overview of potential novel mechanisms of action underlying tumor treating fields-induced cancer cell death and their clinical implications. Int J Radiat Biol. 2021;97(8):1044-1054. doi:10.1080/09553002.2020.1837984 5. Ahmad MA, Al Natour Z, Mustafa F, Rizvi TA. Electrical characterization of normal and cancer cells. IEEE Access. 2018;6:25979-25986. doi:10.1109/access.2018.2830883 6. Gera N, Yang A, Holtzman TS, Lee SX, Wong ET, Swanson KD. Tumor treating fields perturb the localization of septins and cause aberrant mitotic exit. PLoS One. 2015;10(5):e0125269. doi:10.1371/journal.pone.0125269 7. Giladi M, Schneiderman RS, Voloshin T, et al. Mitotic spindle disruption by alternating electric fields leads to improper chromosome segregation and mitotic catastrophe in cancer cells. Sci Rep. 2015;5:1-16. doi:10.1038/srep18046 8. Shams S, Patel CB. Anti-cancer mechanisms of action of therapeutic alternating electric fields (tumor treating fields [TTFields]). J Mol Cell Biol. 2022;14(8):mjac047. doi:10.1093/jmcb/mjac047 9. Voloshin T, Kaynan N, Davidi S, et al. Tumor-treating fields (TTFields) induce immunogenic cell death resulting in enhanced antitumor efficacy when combined with anti-PD-1 therapy. Cancer Immunol Immunother. 2020;69(7):1191-1204. doi:10.1007/s00262-020-02534-7 10. Khagi S, Kotecha R, Gatson NTN, et al. Recent advances in tumor treating fields (TTFields) therapy for glioblastoma. Oncologist. 2025;30(2):oyae227.